Technical Resources

21870 - RPMI 1640, no glutamine

Components	Molecular Weight	Concentration (mg/L)	mM
Amino Acids
Glycine	75.0	10.0	0.13333334
L-Arginine	174.0	200.0	1.1494253
L-Asparagine	132.0	50.0	0.37878788
L-Aspartic acid	133.0	20.0	0.15037593
L-Cystine 2HCl	313.0	65.0	0.20766774
L-Glutamic Acid	147.0	20.0	0.13605443
L-Histidine	155.0	15.0	0.09677419
L-Hydroxyproline	131.0	20.0	0.15267175
L-Isoleucine	131.0	50.0	0.3816794
L-Leucine	131.0	50.0	0.3816794
L-Lysine hydrochloride	146.0	40.0	0.2739726
L-Methionine	149.0	15.0	0.10067114
L-Phenylalanine	165.0	15.0	0.09090909
L-Proline	115.0	20.0	0.17391305
L-Serine	105.0	30.0	0.2857143
L-Threonine	119.0	20.0	0.16806723
L-Tryptophan	204.0	5.0	0.024509804
L-Tyrosine disodium salt	225.0	29.0	0.12888889
L-Valine	117.0	20.0	0.17094018
Vitamins
Biotin	244.0	0.2	8.1967213E-4
Choline chloride	140.0	3.0	0.021428572
D-Calcium pantothenate	477.0	0.25	5.24109E-4
Folic Acid	441.0	1.0	0.0022675737
Niacinamide	122.0	1.0	0.008196721
Para-Aminobenzoic Acid	137.0	1.0	0.00729927
Pyridoxine hydrochloride	206.0	1.0	0.004854369
Riboflavin	376.0	0.2	5.319149E-4
Thiamine hydrochloride	337.0	1.0	0.002967359
Vitamin B12	1355.0	0.005	3.690037E-6
i-Inositol	180.0	35.0	0.19444445
Inorganic Salts
Calcium nitrate (Ca(NO3)2 4H2O)	236.0	100.0	0.42372882
Magnesium Sulfate (MgSO4-7H2O)	246.0	100.0	0.40650406
Potassium Chloride (KCl)	75.0	400.0	5.3333335
Sodium Bicarbonate (NaHCO3)	84.0	2000.0	23.809525
Sodium Chloride (NaCl)	58.0	6000.0	103.44827
Sodium Phosphate dibasic (Na2HPO4-7H2O)	268.0	1512.0	5.641791
Other Components
D-Glucose (Dextrose)	180.0	2000.0	11.111111
Glutathione (reduced)	307.0	1.0	0.0032573289
Phenol Red	376.4	5.0	0.013283741

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