PCR Cloning
 |
A 5-minute, room-temperature reaction requires no gel purification or post-PCR modifications and yields up to 95% recombinants. TOPO® kits are available for general subcloning, long PCR fragments, in vitro transcription, and sequencing. They are also available for expression in E. coli, mammalian cells, and entry into the Gateway® expression system. TA Cloning® kits are designed for cloning directly from a PCR reaction using an overnight ligation step. |
TOPO® TA Cloning
Taq-amplified PCR products with 3´-T overhangs allows for efficient ligation and cloning. Invitrogen's pCR vector backbones offer kanamycin or ampicillin selection and blue/white colony screening.
|
 |
Blunt DNA Cloning
Zero Blunt® TOPO® Cloning Kits eliminates background, making cloning blunt-ended products efficient, fast and fool-proof.
|
|
TA Cloning
| Efficient cloning of Taq-amplified PCR products, 3´-T overhangs for direct ligation, kanamycin or ampicillin selection, and blue/white colony screening. |
|
Selection Guides
-------------------------------------
Feautred Products
Nucleic Acid Purification
Protein Expression