Gateway® Cloning
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Gateway® cloning technology is a universal platform that enables you to shuttle your gene of interest (GOI) into as many expression and functional analysis systems as you‘d like. Orientation and reading frame are maintained with high efficiencies (typically >99%), effectively eliminating the need for secondary sequencing or subcloning after the initial entry clone is made. Gateway® cloning technology also allows you to clone hundreds, or even thousands, of genes at the same time for high-throughput purposes.
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Entry Vectors & Kits
pENTR vectors are available with efficient Directional TOPO® cloning and classic restriction enzyme and ligase cloning.
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Gateway® Vector Conversion Systems
Making your vector Gateway® compatible simplifies your cloning process while enabling access to an extensive collection of vectors.
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Gateway® Recombination Enzymes
BP and LR Clonase™ II enzymes use a single mix format to mediate a robust recombination cloning reaction to create Entry and Expression clones.
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Donor Vectors
| Your PCR insert with attB sites is the ideal substrate for efficient BP recombination cloning into classic pDONR™ vectors, creating an Entry clone. |
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Destination Vectors
An unparalleled selection of Gateway® Destination vectors from expression of proteins in various cells to conducting RNAi studies, crystallography, and protein-protein interaction functional studies.
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MultiSite Gateway®
MultiSite Gateway® technology enables easy, accurate and efficient assembly of multiple DNA fragments in the order and orientation into a single vector.
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Featured Products
LR Clonase™ enzymes
BP Clonase™ Enzymes
Tools
- Download the NEW Gateway® Recombination Cloning Brochure
- Gateway® Reference List
- Gateway® Open Architecture Policy
- Exclusive Interview with James Hartley and Dom Esposito
- Vector NTI Advance™ Software for Gateway® Cloning
- LINNEA™ Pathways